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Home Antibody All anti-IRF7 antibodies

Anti-IRF7 Antibody EPR4718

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307536 Rabbit monoclonal antibody against IRF-7(clone EPR4718) 100ul $325 In Stock
LC400601 IRF7 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(1)
FC(3)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human IRF-7 was used as an immunogen.
Clone NameEPR4718 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100; IHC-Fr: 1:100
Predicted MW Explanation 54 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens interferon regulatory factor 7 (IRF7), transcript variant a
Alternative NameIRF-7H; IRF7A; IRF7B; IRF7C; IRF7H
Database LinkNP_001563
Entrez Gene 3665 Human
Entrez Gene 54123 Mouse
Entrez Gene 293624 Rat
FunctionIRF-7 (interferon regulatory factor 7) is a member of the interferon regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including interferon beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue (1).
Related PathwayTranscription Factors Toll-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - IRF7 antibody [EPR4718]; All lanes : Anti-IRF7 antibody [EPR4718] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Raji cell lysate.Lane 3 : HuT78 cell lysate.Lane 4 : RAW264.7 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 54 kDa.
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TA307536 was used to detect IRF7 in Human tonsil tissue using IHC (Frozen sections). Human tonsil tissue was frozen and fixed with acetone fixed, sample permeabilized with 0.05% Tween and blocked with 6% BSA for 1 hr at room temp. Primary antibody was diluted 1/100 in 2% BSA in TBS/Tween and incubated for 12 hr at 4°C. An Alexa Fluor?488-conjugated Goat anti-rabbit IgG polyclonal(1/25) was used as the secondary antibody. IRF7 staining in green, DAPI in blue.
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Flow Cytometry - Anti-IRF7 antibody [EPR4718]; Flow cytometric analysis of permeabilized Jurkat cells using TA307536 (red) or a rabbit IgG (negative) (green).
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Flow Cytometry - Anti-IRF7 antibody; Overlay histogram showing HeLa cells stained with TA307536 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Flow Cytometry - Anti-IRF7 antibody [EPR4718]; Flow cytometry analysis of Plasmacytoid dendritic cells, staining IRF7 with TA307536. .Cells were fixed in paraformaldehyde and fixed in saponin. The sample was incubated with the primary antibody (1/100 in 2% human serum + 0.5 mM EDTA) for 20 minutes at 4°C. An AlexaFluor?488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.Gating Strategy: FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
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