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Home Antibody All anti-CALB2 antibodies

Anti-CALB2 Antibody EP1798

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307518
  • Rabbit monoclonal antibody against Calretinin (clone EPR1798 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400659) , 20ug
100ul 325 In Stock
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WB(1)
IHC(2)
FC(1)
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Also for CALB2 (NM_001740)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Calretinin was used as an immunogen.
Clone NameEP1798 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:100, ICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens calbindin 2 (CALB2), transcript variant CALB2
Alternative NameCAB29; CAL2; CR
Database LinkNP_001731
FunctionCalretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Calretinin antibody [EP1798]; All lanes : Anti-Calretinin antibody [EP1798] at 1/1000 dilution.Lane 1 : Human fetal Brain lysate.Lane 2 : Mouse brain lysate.Lane 3 : Rat brain lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 29 kDa.Observed band size : 29 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calretinin antibody [EP1798]; TA307518 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calretinin antibody [EP1798]; TA307518 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
FC Image
Flow Cytometry - Anti-Calretinin antibody [EP1798]; Overlay histogram showing SH-SY5Y cells stained with TA307518 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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