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Home Antibody All anti-CD9 antibodies

Anti-CD9 Antibody EPR2949

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307511
  • Rabbit monoclonal antibody against CD9 (clone EPR2949 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400677) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(11)
FC(1)
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Also for CD9 (NM_001769)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human CD9 was used as an immunogen.
Clone NameEPR2949 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:1000; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens CD9 molecule (CD9)
Alternative NameBTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29
Database LinkNP_001760
Entrez Gene 928 Human
Entrez Gene 12527 Mouse
Entrez Gene 24936 Rat
FunctionCD9 belongs to the transmembrane 4 superfamily, also known as tetraspanin (TM4SF) family and is expressed in variety of hematopoietic and epithelial cells (1,2). It can form homodimers or homooligomers as well as heterooligomers with other members of the TM4SF family (3,4). It can trigger platelet activation and aggregation and modulate cell adhesion and migration. In addition, CD9 appears to promote muscle cell fusion and support. Downregualtion of CD9 has been reported to be associated with tumor progression and metastasis (5,6).
Related PathwayES Cell Differentiation/IPSTransmembraneAdult stem cellsEmbryonic stem cellsDruggable Genome Hematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CD9 antibody [EPR2949]; All lanes : Anti-CD9 antibody [EPR2949] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HuT-78 cell lysate.Lane 3 : U87-MG cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 25 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD9 antibody [EPR2949]; TA307511, at a 1/100 dilution, staining CD9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemisty.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD9 antibody [EPR2949]; TA307511, at a 1/100 dilution, staining CD9 in paraffin embedded Human endometrial carcinoma tissue by Immunohistochemisty.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD9 antibody [EPR2949]; TA307511, at a 1/100 dilution, staining CD9 in paraffin embedded Human tonsil tissue by Immunohistochemisty.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Papillary carcinoma of thyroid gland tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Astrocytoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Papillary carcinoma of thyroid gland tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Astrocytoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD9 antibody [EPR2949](TA307511); TA307511 showing positive staining in Normal brain tissue.
FC Image
Flow Cytometry - Anti-CD9 antibody [EPR2949]; Overlay histogram showing Jurkat cells stained with TA307511 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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