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Anti-ENO2 Antibody EPR3378
Also for ENO2 (NM_001975)
|A synthetic peptide corresponding to residues in human Enolase 2 gamma (NSE) was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens enolase 2 (gamma, neuronal) (ENO2)|
|Enolase 2 gamma is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway (1, 2). The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin (2).|
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Western blot - Anti-NSE antibody [EPR3378]; All lanes : Anti-NSE antibody [EPR3378] at 1/1000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : Y79 cell lysate.Lane 4 : U87-MG cell lysate.Lane 5 : HeLa cell lysate.Lane 6 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 47 kDa.