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Anti-ENO2 Antibody EPR3377
Also for ENO2 (NM_001975)
|A synthetic peptide corresponding to residues in human Enolase 2 gamma (NSE) was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:5000 - 1:20000; FC: 1:20 - 1:100; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens enolase 2 (gamma, neuronal) (ENO2)|
|Enolase is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway (1, 2). The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin (2). |
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Western blot - NSE antibody [EPR3377]; All lanes : Anti-NSE antibody [EPR3377] at 1/20000 dilution.Lane 1 : fetal brain lysate.Lane 2 : SH-SY-5Y lysate.Lane 3 : HeLa lysate.Lane 4 : Y79 lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 47 kDa.Observed band size : 47 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NSE antibody [EPR3377]; Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/250-1/500 ab79757.
Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377]; ICC/IF image of ab79757 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79757 at 1/25 dilution overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-NSE antibody [EPR3377](ab79757); Overlay histogram showing HeLa cells stained with ab79757 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.