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Anti-GRN Antibody EPR3781
|A synthetic peptide corresponding to residues in human GRN was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens granulin (GRN)|
|CLN11; GEP; GP88; PCDGF; PEPI; PGRN|
|Granulins (GRNs) are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis (1).|
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Western blot - Granulin antibody [EPR3781]; All lanes : Anti-Granulin antibody [EPR3781] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : HeLa cell lysate.Lane 5 : Human fetal lung tissue lysate.Lane 6 : Human fetal kidney tissue lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 64 kDa.Observed band size : 55,75 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Granulin antibody [EPR3781]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108608 at 1/100.
Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR3781]; ICC/IF image of ab108608 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.