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Home Antibody All anti-GRN antibodies

Anti-GRN Antibody EPR3781

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307483
  • Rabbit monoclonal antibody against GRN(clone EPR3781)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419545) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for GRN (NM_002087)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human GRN was used as an immunogen.
Clone NameEPR3781 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens granulin (GRN)
Alternative NameCLN11; GEP; GP88; PCDGF; PEPI; PGRN
Database LinkNP_002078
Entrez Gene 2896 Human
FunctionGranulins (GRNs) are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis (1).
Related PathwaySecreted ProteinDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Granulin antibody [EPR3781]; All lanes : Anti-Granulin antibody [EPR3781] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : HeLa cell lysate.Lane 5 : Human fetal lung tissue lysate.Lane 6 : Human fetal kidney tissue lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 64 kDa.Observed band size : 55,75 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Granulin antibody [EPR3781]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue using TA307483 at 1/100.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR3781]; ICC/IF image of TA307483 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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