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Anti-H3F3A PHOSPHO Antibody EPR1873
Also for H3F3A (NM_002107)
|A synthetic peptide corresponding to residues surrounding Serine 31 of human Histone H3.3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ChIP: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens H3 histone, family 3A (H3F3A)|
|Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes. |
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Western blot - Histone H3.3 (phospho S31) antibody [EPR1873]; All lanes : Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - ChIP Grade at 1/1000 dilution.Lane 1 : HeLa cell lysate untreated.Lane 2 : HeLa cell lysate treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 15 kDa.
Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - ChIP Grade; .Predicted band size : 15 kDa.This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3.3 (phospho S31) antibody [EPR1873]; Paraffin-embedded human cervical carcinoma labelled with ab92628 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Histone H3.3 (phospho S31) antibody [EPR1873]; Immunofluorescent staining of HeLa cells using ab92628 at 1/100 dilution.