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Home Antibody All anti-HMGB1 antibodies

Anti-HMGB1 Antibody EPR3506

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307477
  • Rabbit monoclonal antibody against HMGB1 (clone EPR3506 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400775) , 20ug Explanation
100ul $325 3-7 Days

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
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Also for HMGB1 (NM_002128)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human HMGB1 was used as an immunogen.
Clone NameEPR3506 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsFC: 1:100, IHC: 1:100 - 250, WB: 1:1000 - 2000,
Predicted MW Explanation 25 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens high mobility group box 1 (HMGB1)
Alternative NameHMG1; HMG3; SBP-1
Database LinkNP_002119
Entrez Gene 3146 Human
Entrez Gene 15289 Mouse
Entrez Gene 25459 Rat
FunctionHigh-mobility group box 1 protein (HMGB1) is a nuclear DNA-binding protein, which also functions as a pleiotropic cytokine, implicated in the pathology of several different immune-mediated diseases (1). As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription. Recent studies identified extracellular HMGB1 as a potent macrophage-activating factor, signaling via the receptor for advanced glycation end-products to induce inflammatory responses. HMGB1 mediates endotoxin lethality, acute lung injury, arthritis induction, activation of macrophages, smooth muscle cell chemotaxis, and epithelial cell barrier dysfunction (2).
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Base excision repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - HMGB1 antibody [EPR3506]; All lanes : Anti-HMGB1 antibody [EPR3506] at 1/1000 dilution.Lane 1 : SKBR3 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Molt4 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 25 kDa.Observed band size : 25 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [EPR3506]; TA307477 at 1/100 dilution staining HMGB1 in Human Liver by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
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