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Anti-HMGB1 Antibody EPR3506
Also for HMGB1 (NM_002128)
|A synthetic peptide corresponding to residues in human HMGB1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:100, IHC: 1:100 - 250, WB: 1:1000 - 2000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens high mobility group box 1 (HMGB1)|
|HMG1; HMG3; SBP-1|
|High-mobility group box 1 protein (HMGB1) is a nuclear DNA-binding protein, which also functions as a pleiotropic cytokine, implicated in the pathology of several different immune-mediated diseases (1). As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription. Recent studies identified extracellular HMGB1 as a potent macrophage-activating factor, signaling via the receptor for advanced glycation end-products to induce inflammatory responses. HMGB1 mediates endotoxin lethality, acute lung injury, arthritis induction, activation of macrophages, smooth muscle cell chemotaxis, and epithelial cell barrier dysfunction (2). |
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Western blot - HMGB1 antibody [EPR3506]; All lanes : Anti-HMGB1 antibody [EPR3506] at 1/1000 dilution.Lane 1 : SKBR3 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Molt4 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 25 kDa.Observed band size : 25 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMGB1 antibody [EPR3506]; ab92310 at 1/100 dilution staining HMGB1 in Human Liver by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.