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Anti-TNC Antibody EPR4219
|A synthetic peptide corresponding to residues in human Tenascin-C was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:1000; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens tenascin C (TNC)|
|150-225; DFNA56; GMEM; GP; HXB; JI; TN; TN-C|
|Tenascin-C is the founding member of a family of extracellular matrix glycoproteins that includes tenascin-X, -R and -W. Tenascin-C interacts with several other extracellular matrix molecules and cell-surface receptors, thus affecting tissue architecture, tissue resilience and cell responses (1). It also modulates cell migration; proliferation; and cellular signaling through induction of pro-inflammatory cytokines and oncogenic signaling molecules, amongst other mechanisms (1). Tenascin-C is highly expressed during embryonic development, tissue repair and in pathological situations such as chronic inflammation and cancer (1). In the adult, Tenascin-C remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle (2).|
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Western blot - Tenascin C antibody [EPR4219]; Anti-Tenascin C antibody [EPR4219] at 1/1000 dilution + Human fetal brain lysate at 10 µg.Predicted band size : 241 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [EPR4219]; TA307471, at 1/100, staining Tenascin C in Human testis by Immunohistochemistry, Paraffin-embedded tissue.
Flow Cytometry - Anti-Tenascin C antibody [EPR4219]; Overlay histogram showing SH-SY5Y cells stained with TA307471 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.