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Home Antibody All anti-TNC antibodies

Anti-TNC Antibody EPR4219

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307471
  • Rabbit monoclonal antibody against Tenascin-C(clone EPR4219)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419496) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for TNC (NM_002160)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Tenascin-C was used as an immunogen.
Clone NameEPR4219 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:1000; IHC-Fr: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens tenascin C (TNC)
Alternative Name150-225; DFNA56; GMEM; GP; HXB; JI; TN; TN-C
Database LinkNP_002151
FunctionTenascin-C is the founding member of a family of extracellular matrix glycoproteins that includes tenascin-X, -R and -W. Tenascin-C interacts with several other extracellular matrix molecules and cell-surface receptors, thus affecting tissue architecture, tissue resilience and cell responses (1). It also modulates cell migration; proliferation; and cellular signaling through induction of pro-inflammatory cytokines and oncogenic signaling molecules, amongst other mechanisms (1). Tenascin-C is highly expressed during embryonic development, tissue repair and in pathological situations such as chronic inflammation and cancer (1). In the adult, Tenascin-C remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle (2).
Related Pathway
Focal Adhesion

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WB Image
Western blot - Tenascin C antibody [EPR4219]; Anti-Tenascin C antibody [EPR4219] at 1/1000 dilution + Human fetal brain lysate at 10 µg.Predicted band size : 241 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [EPR4219]; TA307471, at 1/100, staining Tenascin C in Human testis by Immunohistochemistry, Paraffin-embedded tissue.
FC Image
Flow Cytometry - Anti-Tenascin C antibody [EPR4219]; Overlay histogram showing SH-SY5Y cells stained with TA307471 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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