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Anti-JUN PHOSPHO Antibody EPR2237
|A synthetic peptide corresponding to residues surrounding Threonine 93 of human c-Jun was used as an immunogen. The antibody only detects c-Jun phosphorylated at Threonine 93.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:5000; IP: 1:50
|Does not react with Rat. Is unsuitable for Flow Cyt,ICC/IF or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens jun proto-oncogene (JUN)|
|AP-1; AP1; c-Jun|
|c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5). |
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - c-Jun (phospho T93) antibody [EPR2237]; All lanes : Anti-c-Jun (phospho T93) antibody [EPR2237] at 1/5000 dilution.Lane 1 : NIH/3T3 cell lysates untreated.Lane 2 : NIH/3T3 cell lysates treated with Anisomycin.Lysates/proteins at 10 µg per lane.Secondary.HRP goat anti rabbit at 1/2000 dilution.Predicted band size : 36 kDa.Observed band size : 36 kDa.