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Home Antibody All anti-LRP1 antibodies

Anti-LRP1 Antibody EPR3724

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307451
  • Rabbit monoclonal antibody against LRP1 (clone EPR3724 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400839) , 20ug
100ul 325 In Stock
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WB(1)
IHC(4)
IF(2)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LRP1 was used as an immunogen.
Clone NameEPR3724 IsotypeIgG
Species ReactivityMouse, Rat, Human, Pig ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsIHC-P: 1:100 - 1:250; WB: 1:20000 - 1:50000; IP: 1:50; FC: 1:1000; ICC/IF: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens low density lipoprotein receptor-related protein 1 (LRP1)
Alternative NameA2MR; APOER; APR; CD91; IGFBP3R; LRP; LRP1A; TGFBR5
Database LinkNP_002323
FunctionThe LDL receptor-related protein (LRP1) is a large endocytic receptor that is widely expressed in several tissues. LRP1 is a member of the LDL receptor family that plays diverse roles in various biological processes including lipoprotein metabolism, degradation of proteases, activation of lysosomal enzymes, and cellular entry of bacterial toxins and viruses. LRP1 can recognize more than 30 distinct ligands, bind a large number of cytoplasmic adaptor proteins via determinants located on its cytoplasmic domain in a phosphorylation-specific manner, and can associate with and modulate the activity of other transmembrane receptors such as integrins and receptor tyrosine kinases (1). This multi-talented receptor has been implicated in regulation of platelet derived growth factor receptor activity, integrin maturation and recycling, and focal adhesion disassembly (2).
Related Pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Low Density LRP antibody [EPR3724]; All lanes : Anti-LRP1 [EPR3724] antibody at 1/20000 dilution.Lane 1 : Human PMBC lysate.Lane 2 : A549 lysate.Lane 3 : Mouse brain lysate.Lane 4 : Mouse heart lysate.Lane 5 : Mouse kidney lysate.Lane 6 : Mouse spleen lysate.Lane 7 : Rat brain lysate.Lane 8 : Rat heart lysate.Lane 9 : Rat kidney lysate.Lane 10 : Rat spleen lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Low Density LRP antibody [EPR3724]; TA307451 at 1/100 dilution staining Low Density LRP in formalin-fixed, paraffin-embedded Human liver tissue.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724]; TA307451 showing positive staining in Normal brain tissue.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724]; TA307451 showing positive staining in Normal lung tissue.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724]; TA307451 showing positive staining in Normal placenta tissue.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IF Image
Immunocytochemistry/ Immunofluorescence - Low Density LRP antibody [EPR3724]; TA307451 at 1/100 dilution staining Low Density LRP in HEPG2 cells by Immunofluorescence.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [EPR3724]; ICC/IF image of LRP1 staining on rat mixed glia culture using TA307451 (1:200). The cells were fixed using paraformaldehyde. The cells were then permeabilised using 0.1% TritonX in 0.1% PBS. Non-specific protein was blocked using 10% donkey serum at 24°C for 1 hour. Ab92544 was diluted(1:200) using 0.1% TritonX with 0.1% PBS and 10% donkey serum and the cells were incubated for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to Rabbit IgG conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
FC Image
Flow Cytometry - Anti-LRP1 antibody [EPR3724]; Overlay histogram showing Jurkat cells stained with TA307451 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>

 

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