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Anti-MBD1 Antibody EPR3564
Also for MBD1 (NM_002384)
|A synthetic peptide corresponding to residues in human MBD1 was used as an immunogen|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500 - 1:1000; IP: 1:20; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 4|
|CXXC3; PCM1; RFT|
|Methyl-CpG-binding domain protein 1 (MBD1) is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides (1). Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. It is also believed to form a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (2).|
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Western blot - MBD1 antibody [EPR3564]; All lanes : Anti-MBD1 antibody [EPR3564] at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A431 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 67 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MBD1 antibody [EPR3564]; ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - MBD1 antibody [EPR3564]; ab108510, at 1/100 dilution, staining MBD1 in MCF7 cells by Immunofluorescence.