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Anti-MGMT Antibody EPR4397
Also for MGMT (NM_002412)
|A synthetic peptide corresponding to residues in human MGMT was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens O-6-methylguanine-DNA methyltransferase (MGMT)|
|O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase|
|O(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein that protects tissues against toxic and carcinogenic effects of alkylating agents (1). It is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA, and repairs alkylated guanine in DNA; a suicide reaction, because the enzyme is irreversibly inactivated (2). MGMT is ubiquitously expressed in normal human tissues but is overexpressed in all types of human tumors, including colon cancer, breast cancer, leukemia, lymphomas, and myeloma. Although tumor cells have many mechanisms of resistance to alkylating agents, the expression of MGMT in tumors has a protective effect against cell death induced by alkylating agents, showing a strong correlation between MGMT activity and tumor drug resistance (3).|
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Western blot - MGMT antibody [EPR4397]; All lanes : Anti-MGMT antibody [EPR4397] at 1/1000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : Jurkat ell lysate.Lane 3 : HeLa cell lysate.Lane 4 : HT29 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 22 kDa.Observed band size : 23 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MGMT antibody [EPR4397]; ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MGMT antibody [EPR4397]; ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.