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Home Antibody All anti-MGMT antibodies

Anti-MGMT Antibody EPR4397

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SKU Description Amount Price Availability*  
TA307447
  • Rabbit monoclonal antibody against MGMT(clone EPR4397)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC432155) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(2)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human MGMT was used as an immunogen.
Clone NameEPR4397 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameHomo sapiens O-6-methylguanine-DNA methyltransferase (MGMT)
Alternative NameO6-methylguanine-DNA methyltransferase; OTTHUMP00000020741; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase
Database LinkNP_002403
Entrez Gene 4255 Human
FunctionO(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein that protects tissues against toxic and carcinogenic effects of alkylating agents (1). It is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA, and repairs alkylated guanine in DNA; a suicide reaction, because the enzyme is irreversibly inactivated (2). MGMT is ubiquitously expressed in normal human tissues but is overexpressed in all types of human tumors, including colon cancer, breast cancer, leukemia, lymphomas, and myeloma. Although tumor cells have many mechanisms of resistance to alkylating agents, the expression of MGMT in tumors has a protective effect against cell death induced by alkylating agents, showing a strong correlation between MGMT activity and tumor drug resistance (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - MGMT antibody [EPR4397]; All lanes : Anti-MGMT antibody [EPR4397] at 1/1000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : Jurkat ell lysate.Lane 3 : HeLa cell lysate.Lane 4 : HT29 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 22 kDa.Observed band size : 23 kDa .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MGMT antibody [EPR4397]; TA307447, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MGMT antibody [EPR4397]; TA307447, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.
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