OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
 Home All anti-MGMT antibodies

Anti-MGMT Antibody EPR4397

div

Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA307447
  • Rabbit monoclonal antibody against MGMT(clone EPR4397)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC432155) , 20ug
 100ul $325 3-7 Days
TA802519S
  • Purified GAPDH mouse monoclonal antibody (Loading control), clone 2D9
 30ul
$100
$50*		 In Stock
Add to Shopping Cart
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
WB(1)
IHC(1)
spacer
Also for MGMT (NM_002412)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human MGMT was used as an immunogen.
Clone NameEPR4397 IsotypeRabbit IgG
Species ReactivityHuman Concentration
Guaranteed Application *WB, IHC Suggested DilutionsIHC: 1:250 - 500, WB: 1:1,000 - 10,000,
BufferStore at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Reference Data

Target NameHomo sapiens O-6-methylguanine-DNA methyltransferase (MGMT)
Alternative NameO6-methylguanine-DNA methyltransferase; OTTHUMP00000020741; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase
Database LinkNP_002403
FunctionO(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein that protects tissues against toxic and carcinogenic effects of alkylating agents (1). It is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA, and repairs alkylated guanine in DNA; a suicide reaction, because the enzyme is irreversibly inactivated (2). MGMT is ubiquitously expressed in normal human tissues but is overexpressed in all types of human tumors, including colon cancer, breast cancer, leukemia, lymphomas, and myeloma. Although tumor cells have many mechanisms of resistance to alkylating agents, the expression of MGMT in tumors has a protective effect against cell death induced by alkylating agents, showing a strong correlation between MGMT activity and tumor drug resistance (3).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis on (A) MCF-7, (B) Jurkat, (C) HeLa, and (D) HT-29 cell lysates using anti-MGMT RabMAb .
IHC Image
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma tissue using anti-MGMT RabMAb .

 

spacer
Inc 5000 Healthcare Company Copyright © 2013 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title