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Anti-NBN PHOSPHO Antibody EPR2470Y
|A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens nibrin (NBN)|
|AT-V1; AT-V2; ATV; NBS; NBS1; P95|
|Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. p95/NBS1 is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. Phosphorylation of p95/NBS1 mediated by gamma-radiation, but not that induced by hydroxyurea or ultraviolet light, was markedly reduced in ATM cells. Phosphorylation of p95/NBS1 by ATM is critical for certain responses of human cells to DNA damage (1). p95/NBS1 is part of a protein complex that is involved in responses to DNA double-strand breaks. Observations link ATM and p95/NBS1 in a common signaling pathway and provide an explanation for phenotypic similarities in NBS and ATM diseases (2). Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. NBS1 is essential for homologous recombination-mediated repair in higher vertebrate cells and the disruption of p95/NBS1 reduces gene conversion and sister chromatid exchanges (3). |
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Western blot - p95 NBS1 (phospho S432) antibody [EPR2470Y]; All lanes : Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y] at 1/500 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with doxorubincin.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p95 NBS1 (phospho S432) antibody [EPR2470Y]; ab75778 at 1/100-1/250 dilution staining p95 NBS1 in human testis by Immunohistochemistry, Paraffin-embedded tissue.