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Anti-NBN PHOSPHO Antibody EP178
|A phospho-specific peptide corresponding to residues surrounding Serine 343 in human Nibrin/NBS1 was used as an immunogen. The antibody only detects Nibrin/NBS1 phosphorylated on Serine 343.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500 - 1:1000; IP: 1:50; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens nibrin (NBN)|
|AT-V1; AT-V2; ATV; NBS; NBS1; P95|
Entrez Gene 4683 Human
|Nibrin (Nijmegen breakage syndrome protein 1, NBS1) contains 2 modules found in cell cycle check point; a forkhead-associated domain and a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 and Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair and telomere maintenance in eukaryotic cells. Mutation in Nibrin and Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) and Ataxia-Telangiectasia- like disorder (ATLD) (2-4). Nibrin is phosphorylated at Serine 343 by ATM. The in vivo modification of these residues is essential for the cellular response to DNA damage, including an S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation (5). |
|Druggable Genome Homologous recombination|
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Western blot - p95 NBS1 (phospho S343) antibody [EP178]; All lanes : Anti-p95 NBS1 (phospho S343) antibody [EP178] at 1/100 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with Etopside.Lysates/proteins at 10 ug per lane.Predicted band size : 84 kDa.Observed band size : 95 kDa .