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Anti-P2RX7 Antibody EPR4723
Also for P2RX7 (NM_002562)
|A synthetic peptide corresponding to residues in the C-terminus of human P2RX7 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1|
|P2RX7 belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria (1).|
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Western blot - P2RX7 antibody [EPR4723]; All lanes : Anti-P2RX7 antibody [EPR4723] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : A431 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Human fetal kidney tissue lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 69 kDa.Observed band size : 75 kDa .
Immunocytochemistry/ Immunofluorescence - P2RX7 antibody [EPR4723]; ab109246, at 1/100, staining P2RX7 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-P2RX7 antibody [EPR4723]; Overlay histogram showing HeLa cells stained with ab109246 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.