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Anti-PCNA Antibody EPR3822
Also for PCNA (NM_002592)
|A synthetic peptide corresponding to residues near the C terminus in human PCNA was used as an immunogen.|
||Lot dependent; please refer to CoA along with shipment
||FC: 1:10, IHC: 1:50 - 100, WB: 1:1,000 - 5,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1|
|Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2). |
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Western blot - PCNA antibody [EPR3822]; All lanes : Anti-PCNA antibody [EPR3822] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 29 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody [EPR3822]; TA307433, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody [EPR3822]; TA307433, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty.