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Home Antibody All anti-PCNA antibodies

Anti-PCNA Antibody EPR3822

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307433
  • Rabbit monoclonal antibody against PCNA (clone EPR3822 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400929) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)
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Also for PCNA (NM_002592)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C terminus in human PCNA was used as an immunogen.
Clone NameEPR3822 IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsFC: 1:10, IHC: 1:50 - 100, WB: 1:1,000 - 5,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameMGC8367
Database LinkNP_002583
FunctionProliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).
Related Pathway
Cell cycle

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PCNA antibody [EPR3822]; All lanes : Anti-PCNA antibody [EPR3822] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 29 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody [EPR3822]; TA307433, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody [EPR3822]; TA307433, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty.

 

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