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Anti-PDGFRB PHOSPHO Antibody EPR2476
Also for PDGFRB (NM_008809)
|A phospho specific peptide corresponding to residues surrounding tyrosine 1021 of human PDGF Receptor beta was used as an immunogen. This antibody only detects PDGF Receptor beta phosphorylated at tyrosine 1021.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|Does not react with Rat, Human. Is unsuitable for ICC or IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for ICC or IHC-P.
|Mus musculus platelet derived growth factor receptor, beta polypeptide (Pdgfrb), transcript variant 2|
|AI528809; CD140b; Pdgfr; PDGFR-1|
|Human platelet derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signaling cascades leading to cell growth and proliferation. The two major isoforms that exist are PDGF Receptor beta and PDGF Receptor alpha. PDGF Receptor beta can only bind to two PDGF isoforms, PDGF-beta and PDGF-delta (1, 2). Upon ligand binding, the receptor undergoes dimerization, resulting in autophosphorylation to allow activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase, Grb2, Src, etc. (3). Phosphorylation of the PDGF Receptor at Tyrosine 1021 is required for the stable association of PLC gamma to the receptor's C-terminus, the production of inositol phosphates, and initiation of the maximal mitogenic response (4). A chromosomal aberration involving the PDGF Receptor beta is found in a form of chronic myelomonocytic leukemia (5).|
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Western blot - Anti-PDGF Receptor beta (phospho Y1021) antibody [EPR2476]; All lanes : Anti-PDGF Receptor beta (phospho Y1021) antibody [EPR2476] at 1/50000 dilution.Lane 1 : NIH/3T3 untreated .Lane 2 : NIH/3T3 treated with Pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 190 kDa.