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Anti-PDPK1 Antibody EPR245
Also for PDPK1 (NM_002613)
|A synthetic peptide corresponding to residues in N-terminus of human PDK1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 1|
|PDK1; PDPK2; PRO0461|
|PDK1 (3-Phosphoinositide-dependent protein kinase 1) is a serine/threonine protein kinase known to phosphorylate and activate various signal transduction pathways: Akt/PKB (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC, and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1, 2). Unlike other phosphorylation sites on PDK1, only phosphorylation on the activation loop at Ser241 is necessary for PDK1 activity; a single mutation to Ala abolishes its activity (3). PDK1 can also autophosphorylate Ser241, leading to its own activation (3).|
Signaling by GPCR
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Western blot - PDPK1 antibody [EPR245]; All lanes : Anti-PDPK1 antibody [EPR245] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : PC12 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 63 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 antibody [EPR245]; ab109253, at a 1/500 dilution, staining PDPK1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.