Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 1
Synonyms:
PDK1; PRO0461
Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 241 of human PDK1 was used as an immunogen. This antibody only detects PDK1 phosphorylated at Serine 241.
Buffer
Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine/threonine protein kinase known to phosphorylate and activate various signal transduction pathways: Akt/PKB (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC, and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1, 2). Unlike other phosphorylation sites on PDK1, only phosphorylation on the activation loop at Ser241 is necessary for PDK1 activity; a single mutation to Ala abolishes its activity (3). PDK1 can also autophosphorylate Ser241, leading to its own activation (3).
Related Pathway
Focal Adhesion
Hemostasis
Signaling by GPCR
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