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Anti-POU5F1 Antibody EPR2053(2)
Also for POU5F1 (NM_002701)
|A synthetic peptide corresponding to residues in the N-terminus of human Oct-4A was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for ICC or IHC-P.
|Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1|
|Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4|
|Octamer transcription factor-4 (Oct-4) is a homeodomain transcription factor in the POU transcription factor family that specifically binds to the octamer motif (5’-ATTTCAT-3’) (1). It is critical for early embryogenesis and for embryonic stem cell pluripotency, expressed mainly in the developing brain (1). Oct-4 is a master regulator that affects the fate of pluripotent stem cells and germ cell precursors (2). For instance, the downregulation of Oct-4 triggers embryonic stem cell differentiation, simultaneous with gastrulation (3). It is also used as a marker for germ cell tumor diagnosis (4). Oct-4 exists as two different isoforms, Oct-4A and Oct-4B. Oct-4A can sustain embryonic stem cell self-renewal, whereas Oct-4B is localized in the cytoplasm (5).|
Wnt Signaling Pathway
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Western blot - Oct4 antibody [EPR2053(2)]; All lanes : Anti-Oct4 antibody [EPR2053(2)] at 1/1000 dilution.Lane 1 : Human seminoma lysate.Lane 2 : NCCIT cell lysate.Lysates/proteins at 10 µg per lane.Performed under reducing conditions.Predicted band size : 39 kDa.