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Anti-PRKCG PHOSPHO Antibody EP2730Y
Also for PRKCG (NM_002739)
|A phospho specific peptide corresponding to residues surrounding threonine 514 of human PKC gamma was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens protein kinase C, gamma (PRKCG)|
|PKC-gamma; PKCC; PKCG; SCA14|
|Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types and, to date, the cloning of seven mammalian PKC genes encoding eight distinct isoforms has been reported. PKC gamma (PKCG) is calcium, diacyglycerol, and phorbol esters activated, serine- and threonine-specific enzyme. PKC gamma expression is specific to neurons and it plays an important role in neural signal transduction (1). After activation by phorbol, it moves to cytoplasm, where it shown to regulate intracellular communication in epithelial cells. PKC has three phosphorylation sites in its kinase domain, Thr 514, 655, and 674. Phosphorylations of these three sites are critical for full activation of PKC in response to various stimulants (2). PKC gamma is phosphorylated at Thr 514 by PDK1. PKC gamma is required for long term potentiation (LTP) and long term depression (LTD). Defect in PKC gamma has been linked to neurodegenerative disorder spinocerebellar ataxia-14 (SCA14) (3).|
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Western blot - PKC gamma (phospho T514) antibody [EP2730Y]; All lanes : Anti-PKC gamma (phospho T514) antibody [EP2730Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate treated with LP.Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 78 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC gamma (phospho T514) antibody [EP2730Y]; TA307413 at 1/100 dilution staining PKC gamma (phospho T514) in paraffin-embedded Human brain tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - PKC gamma (phospho T514) antibody [EP2730Y]; TA307413 at 1/100 dilution staining PKC gamma (phospho T514) in Sh-Sy-5Y cells by Immunofluorescence.