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Anti-PRKD1 Antibody EPR1492(2)
Also for PRKD1 (NM_002742)
|A synthetic peptide corresponding to residues in human PKC mu / PKD was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens protein kinase D1 (PRKD1)|
|PKC-MU; PKCM; PKD; PRKCM|
|PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. Mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).|
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Western blot - PKC mu antibody [EPR1492(2)]; All lanes : Anti-PKC mu antibody [EPR1492(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 102 kDa.