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Home Antibody All anti-RPA1 antibodies

Anti-RPA1 Antibody EPR3473

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307404
  • Rabbit monoclonal antibody against RPA 70 (clone EPR3473 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401030) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
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Also for RPA1 (NM_002945)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human RPA 70 was used as an immunogen.
Clone NameEPR3473 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:50; FC: 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC/IF or IHC-P.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for ICC/IF or IHC-P.

Reference Data

Target NameHomo sapiens replication protein A1, 70kDa (RPA1)
Alternative NameHSSB; MST075; REPA1; RF-A; RP-A; RPA70
Database LinkNP_002936
Entrez Gene 6117 Human
FunctionReplication protein A (RPA) is a single-stranded DNA-binding (ssDNA) protein involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination. It is found to be essential for replication of the papovavirus SV40 (1). RPA is a complex of three polypeptides of 70, 34, and 13 kDa (2). The DNA-binding activity may reside exclusively on the 70-kDa subunit RPA 70. The 70-kDa subunit of RPA (RPA 70) is composed of three functional domains consisting of an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions (3).
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repairHomologous recombination

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-RPA70 antibody [EPR3473]; All lanes : Anti-RPA70 antibody [EPR3473] at 1/10000 dilution.Lane 1 : A431 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 68 kDa.Observed band size : 70 kDa .

 

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