A phospho specific peptide corresponding to residues surrounding threonine 21 of human RPA32 was used as an immunogen. This antibody only detects RPA32 phosphorylated at threonine 21.
Buffer
Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that plays essential roles in nucleic acid metabolism, including DNA replication, nucleotide excision repair, and homologous recombination. It is a complex of three polypeptides of molecular mass 70, 32, and 14 kDa (RPA70, RPA32, and RPA14). RPA is a phosphorylation target for DNA-dependent protein kinase (DNA-PK) and likely the ataxia telangiectasia-mutated gene (ATM) protein kinase. The middle subunit, RPA32, is phosphorylated in a cell cycle-dependent manner, which begins in parallel to the degradation of DNA to high molecular weight fragments; it slowly continues until late apoptosis. RPA32 has been reported to be a useful prognostic indicator in colon cancer patients and an attractive therapeutic target for regulation by tumor suppressors or other proteins involved in the control of cell proliferation (4).
Related Pathway
Cell cycle
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