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Anti-RPA2 PHOSPHO Antibody EPR2846(2)
Also for RPA2 (NM_002946)
|A phospho specific peptide corresponding to residues surrounding threonine 21 of human RPA32 was used as an immunogen. This antibody only detects RPA32 phosphorylated at threonine 21.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: 1:500 - 1:1000; WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens replication protein A2, 32kDa (RPA2), transcript variant 1|
|Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that plays essential roles in nucleic acid metabolism, including DNA replication, nucleotide excision repair, and homologous recombination. It is a complex of three polypeptides of molecular mass 70, 32, and 14 kDa (RPA70, RPA32, and RPA14). RPA is a phosphorylation target for DNA-dependent protein kinase (DNA-PK) and likely the ataxia telangiectasia-mutated gene (ATM) protein kinase. The middle subunit, RPA32, is phosphorylated in a cell cycle-dependent manner, which begins in parallel to the degradation of DNA to high molecular weight fragments; it slowly continues until late apoptosis. RPA32 has been reported to be a useful prognostic indicator in colon cancer patients and an attractive therapeutic target for regulation by tumor suppressors or other proteins involved in the control of cell proliferation (4).|
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Western blot - RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]; All lanes : Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] at 1/50000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with Camptothecin.Lysates/proteins at 10 µg per lane.Predicted band size : 29 kDa.Observed band size : 32 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]; Immunohistochemical analysis of RPA32/RPA2 in paraffin embedded Human colon tissue, using ab109394 at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]; Immunofluorescence analysis of Human U2OS cells, staining RPA32/RPA2 (phospho T21) with ab109394. Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA+ FBS for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An AlexaFluor?488-conjugated anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.