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Anti-RPA2 PHOSPHO Antibody EPR2846(2)


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against RPA32 Phospho (pT21)(clone EPR2846(2)) (Phospho-Specific)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 21 of human RPA32 was used as an immunogen. This antibody only detects RPA32 phosphorylated at threonine 21.
Clone NameEPR2846(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: 1:500 - 1:1000; WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500
Predicted MW Explanation 29 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens replication protein A2, 32kDa (RPA2), transcript variant 1
Alternative NameREPA2; RP-A p32; RP-A p34; RPA32
Database LinkNP_002937
Entrez Gene 6118 Human
Entrez Gene 19891 Mouse
Entrez Gene 59102 Rat
FunctionReplication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that plays essential roles in nucleic acid metabolism, including DNA replication, nucleotide excision repair, and homologous recombination. It is a complex of three polypeptides of molecular mass 70, 32, and 14 kDa (RPA70, RPA32, and RPA14). RPA is a phosphorylation target for DNA-dependent protein kinase (DNA-PK) and likely the ataxia telangiectasia-mutated gene (ATM) protein kinase. The middle subunit, RPA32, is phosphorylated in a cell cycle-dependent manner, which begins in parallel to the degradation of DNA to high molecular weight fragments; it slowly continues until late apoptosis. RPA32 has been reported to be a useful prognostic indicator in colon cancer patients and an attractive therapeutic target for regulation by tumor suppressors or other proteins involved in the control of cell proliferation (4).
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repairHomologous recombination

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Western blot - RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]; All lanes : Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] at 1/50000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with Camptothecin.Lysates/proteins at 10 ug per lane.Predicted band size : 29 kDa.Observed band size : 32 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]; Immunohistochemical analysis of RPA32/RPA2 in paraffin embedded Human colon tissue, using TA307401 at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)]; Immunofluorescence analysis of Human U2OS cells, staining RPA32/RPA2 (phospho T21) with TA307401. Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA+ FBS for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An AlexaFluor?488-conjugated anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.


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