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Anti-S100A9 Antibody EPR3555
Also for S100A9 (NM_002965)
|A synthetic peptide corresponding to residues in human MRP14/S100-A9 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:5000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens S100 calcium binding protein A9 (S100A9)|
|60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14|
|S100-A9 (MRP14) is a member of the S100 family proteins containing two EF-hand calcium-binding motifs (1). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation (2). S100-A9 forms a heterodimer, Calprotectin, with S100-A8 in a calcium-dependent manner. Calprotectin is a major calcuim- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes (3). S100-A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis (2). |
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Western blot - S100A9 antibody [EPR3555]; All lanes : Anti-S100A9 antibody [EPR3555] at 1/1000 dilution.Lane 1 : Breast cancer lysate.Lane 2 : Human tonsil lysate.Lane 3 : Human spleen lysate.Lane 4 : Peripheral blood lymphocytes lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 13 kDa.Observed band size : 14 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A9 antibody [EPR3555]; ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A9 antibody [EPR3555]; ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR3555]; ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.