OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-S100A9 antibodies

Anti-S100A9 Antibody EPR3555

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307398
  • Rabbit monoclonal antibody against MRP14/S100-A9 (clone EPR3555 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401037) , 20ug
100ul $325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(2)
IF(1)
spacer
Also for S100A9 (NM_002965)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human MRP14/S100-A9 was used as an immunogen.
Clone NameEPR3555 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens S100 calcium binding protein A9 (S100A9)
Alternative Name60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14
Database LinkNP_002956
FunctionS100-A9 (MRP14) is a member of the S100 family proteins containing two EF-hand calcium-binding motifs (1). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation (2). S100-A9 forms a heterodimer, Calprotectin, with S100-A8 in a calcium-dependent manner. Calprotectin is a major calcuim- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes (3). S100-A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis (2).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - S100A9 antibody [EPR3555]; All lanes : Anti-S100A9 antibody [EPR3555] at 1/1000 dilution.Lane 1 : Breast cancer lysate.Lane 2 : Human tonsil lysate.Lane 3 : Human spleen lysate.Lane 4 : Peripheral blood lymphocytes lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 13 kDa.Observed band size : 14 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A9 antibody [EPR3555]; TA307398, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A9 antibody [EPR3555]; TA307398, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR3555]; ICC/IF image of TA307398 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title