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Anti-SATB1 Antibody EPR3895


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against SATB1(clone EPR3895)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418976) , 20ug Explanation
100ul 325 3-7 Days
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Also for SATB1 (NM_002971)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus in human SATB1 was used as an immunogen.
Clone NameEPR3895 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100; FC: 1:1000
Predicted MW Explanation 85 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens SATB homeobox 1 (SATB1), transcript variant 1
Alternative NameDNA-binding protein SATB1; special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's); SATB homeobox 1
Database LinkNP_002962
Entrez Gene 6304 Human
Entrez Gene 20230 Mouse
FunctionSpecial AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and nuclear transcription factor which integrates higher-order chromatin architecture with gene regulation (1). SATB1 specifically binds to nuclear matrix/scaffold-associating DNAs (MARs/SARs), which is made of AT-rich DNA sequences with one strand consisting of ATC sequences. Studies have shown that in cooperation with promyelocytic leukemia protein (PML), SATB1 and PML function as a regulatory complex which controls transcription through restructuring dynamic chromatin-loop architecture. SATB1 interacts with PML to organize the MHC class-I locus into distinct higher-order chromatin loops by anchoring MARs to nuclear matrix at fixed distances (2). As a key player in the immune system, SATB1 has been linked to regulating gene expression during the differentiation and activation of T cells. In cancer, SATB1 has been shown to reprogram chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis (3).
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SATB1 antibody [EPR3895]; All lanes : Anti-SATB1 antibody [EPR3895] at 1/1000 dilution.Lane 1 : Jurkat lysate.Lane 2 : Mouse thymus lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 85 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB1 antibody [EPR3895]; TA307395, at a 1/50 dilution, staining SATB1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB1 antibody [EPR3895]; TA307395, at a 1/50 dilution, staining SATB1 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody [EPR3895]; TA307395 staining SATB1 in murine primary thymocytes (isolated from a 21 day old male mouse) by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.1% Triton X-100 for 5 minutes, blocked with 2% BSA for 1 hour at 25°C and then incubated with TA307395 at a 1/200 dilution for 3 hours at 25°C. The secondary used was a 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
FC Image
Flow Cytometry - Anti-SATB1 antibody [EPR3895]; Overlay histogram showing Jurkat cells stained with TA307395 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


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