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Anti-SATB1 Antibody EPR3895
Also for SATB1 (NM_002971)
|A synthetic peptide corresponding to residues near the N-terminus in human SATB1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens SATB homeobox 1 (SATB1), transcript variant 1|
|DNA-binding protein SATB1; special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's); SATB homeobox 1|
|Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and nuclear transcription factor which integrates higher-order chromatin architecture with gene regulation (1). SATB1 specifically binds to nuclear matrix/scaffold-associating DNAs (MARs/SARs), which is made of AT-rich DNA sequences with one strand consisting of ATC sequences. Studies have shown that in cooperation with promyelocytic leukemia protein (PML), SATB1 and PML function as a regulatory complex which controls transcription through restructuring dynamic chromatin-loop architecture. SATB1 interacts with PML to organize the MHC class-I locus into distinct higher-order chromatin loops by anchoring MARs to nuclear matrix at fixed distances (2). As a key player in the immune system, SATB1 has been linked to regulating gene expression during the differentiation and activation of T cells. In cancer, SATB1 has been shown to reprogram chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis (3).|
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Western blot - SATB1 antibody [EPR3895]; All lanes : Anti-SATB1 antibody [EPR3895] at 1/1000 dilution.Lane 1 : Jurkat lysate.Lane 2 : Mouse thymus lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 85 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB1 antibody [EPR3895]; ab92307, at a 1/50 dilution, staining SATB1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB1 antibody [EPR3895]; ab92307, at a 1/50 dilution, staining SATB1 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody [EPR3895]; ab92307 staining SATB1 in murine primary thymocytes (isolated from a 21 day old male mouse) by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.1% Triton X-100 for 5 minutes, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab92307 at a 1/200 dilution for 3 hours at 25°C. The secondary used was a 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
Flow Cytometry - Anti-SATB1 antibody [EPR3895]; Overlay histogram showing Jurkat cells stained with ab92307 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.