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Home Antibody All anti-TFF1 antibodies

Anti-TFF1 Antibody EPR3972

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307378
  • Rabbit monoclonal antibody against TFF1(clone EPR3972)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418822) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(5)
IF(1)
Assay(1)
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Also for TFF1 (NM_003225)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

Immunogen A synthetic peptide corresponding to residues in human TFF1 was used as an immunogen.
Clone NameEPR3972 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens trefoil factor 1 (TFF1)
Alternative NameBCEI; D21S21; HP1.A; HPS2; pNR-2; pS2
Database LinkNP_003216
FunctionTrefoil factor 1 (TFF1), a member of the trefoil factor family, is a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents (1). It belongs to a family of abundant GI3 peptides with a distinct 3-loop structure formed by a highly conserved motif of cysteine disulfide bonds, which confer them with luminal stability (2). TFF peptides, closely associated with mucins, are mainly synthesized and secreted by mucin-secreting epithelial cells lining the gastrointestinal tract (3). It is specifically found in the stomach, with highest expression levels in the upper gastric mucosal cells (1). TFF1 is most strongly expressed in pancreas, large bowel, and breast cancer (1). In breast cancer, its regulation by estrogen is an indicator of estrogen receptor (ER) functionality (4).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Estrogen Inducible Protein pS2 antibody [EPR3972]; All lanes : Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] at 1/1000 dilution.Lane 1 : Human stomach lysate.Lane 2 : MCF-7 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 9 kDa.
Assay Image
Other-Anti-Estrogen Inducible Protein pS2 antibody [EPR3972](TA307378); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Inducible Protein pS2 antibody [EPR3972]; TA307378 staining Estrogen Inducible Protein pS2 in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue (A) and Human stomach tissue (B) at 1/100 dilution. Detection: DAB staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Inducible Protein pS2 antibody [EPR3972](TA307378); TA307378 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Inducible Protein pS2 antibody [EPR3972](TA307378); TA307378 showing negative staining in Normal breast tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Inducible Protein pS2 antibody [EPR3972](TA307378); TA307378 showing negative staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Inducible Protein pS2 antibody [EPR3972](TA307378); TA307378 showing positive staining in Ductal infiltrating breast carcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-Estrogen Inducible Protein pS2 antibody [EPR3972](TA307378); ICC/IF image of TA307378 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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