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Home Antibody All anti-TFRC antibodies

Anti-TFRC Antibody EPR4012

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307375
  • Rabbit monoclonal antibody against CD71(clone EPR4012)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418818) , 20ug Explanation
100ul 325 In Stock
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WB(1)
FC(1)
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Also for TFRC (NM_003234)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus cytoplasmic domain of human CD71 was used as an immunogen.
Clone NameEPR4012 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens transferrin receptor (TFRC), transcript variant 1
Alternative NameCD71; p90; T9; TFR; TFR1; TR; TRFR
Database LinkNP_003225
Entrez Gene 7037 Human
FunctionTransferrin receptor protein 1 (TfR1, CD71) is a plasma membrane transport glycoprotein composed of disulfide-linked polypeptide chains. It belongs to the peptidase m28 family. CD71 is a cell surface membrane protein, with the highest level of expression being in lymphocytes, placenta and neoplastic cells. CD71 is primarily involved in iron homeostasis by regulating cellular iron uptake in interaction with the HFE protein. It is also crucial in iron transportation from mother to fetus (1).
Related PathwaySecreted ProteinES Cell Differentiation/IPSTransmembraneProteaseDruggable Genome EndocytosisHematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Transferrin Receptor antibody [EPR4012]; All lanes : Anti-Transferrin Receptor antibody [EPR4012] at 1/1000 dilution.Lane 1 : Human placenta cell lysate.Lane 2 : TF-1 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : A549 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Lanes 1 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 84 kDa.Observed band size : 90 kDa .
FC Image
Flow Cytometry-Anti-Transferrin Receptor antibody [EPR4012](TA307375); Overlay histogram showing Jurkat cells stained with TA307375 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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