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Anti-TFRC Antibody EPR4012


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA307375 Rabbit monoclonal antibody against CD71(clone EPR4012) 100ul $325 In Stock
LC418818 TFRC HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus cytoplasmic domain of human CD71 was used as an immunogen.
Clone NameEPR4012 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens transferrin receptor (TFRC), transcript variant 1
Alternative NameCD71; p90; T9; TFR; TFR1; TR; TRFR
Database LinkNP_003225
Entrez Gene 7037 Human
FunctionTransferrin receptor protein 1 (TfR1, CD71) is a plasma membrane transport glycoprotein composed of disulfide-linked polypeptide chains. It belongs to the peptidase m28 family. CD71 is a cell surface membrane protein, with the highest level of expression being in lymphocytes, placenta and neoplastic cells. CD71 is primarily involved in iron homeostasis by regulating cellular iron uptake in interaction with the HFE protein. It is also crucial in iron transportation from mother to fetus (1).
Related PathwaySecreted ProteinES Cell Differentiation/IPSTransmembraneProteaseDruggable Genome EndocytosisHematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Transferrin Receptor antibody [EPR4012]; All lanes : Anti-Transferrin Receptor antibody [EPR4012] at 1/1000 dilution.Lane 1 : Human placenta cell lysate.Lane 2 : TF-1 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : A549 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Lanes 1 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 84 kDa.Observed band size : 90 kDa .

FC Image
Flow Cytometry-Anti-Transferrin Receptor antibody(TA307375); Overlay histogram showing Jurkat cells stained with TA307375 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in Jurkat cells under the same conditions.


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