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Anti-TFRC Antibody EPR4012
Also for TFRC (NM_003234)
|A synthetic peptide corresponding to residues near the C-terminus cytoplasmic domain of human CD71 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens transferrin receptor (TFRC), transcript variant 1|
|CD71; p90; T9; TFR; TFR1; TR; TRFR|
|Transferrin receptor protein 1 (TfR1, CD71) is a plasma membrane transport glycoprotein composed of disulfide-linked polypeptide chains. It belongs to the peptidase m28 family. CD71 is a cell surface membrane protein, with the highest level of expression being in lymphocytes, placenta and neoplastic cells. CD71 is primarily involved in iron homeostasis by regulating cellular iron uptake in interaction with the HFE protein. It is also crucial in iron transportation from mother to fetus (1).|
Hematopoietic cell lineage
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Western blot - Transferrin Receptor antibody [EPR4012]; All lanes : Anti-Transferrin Receptor antibody [EPR4012] at 1/1000 dilution.Lane 1 : Human placenta cell lysate.Lane 2 : TF-1 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Lanes 1 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 84 kDa.Observed band size : 90 kDa .
Flow Cytometry-Anti-Transferrin Receptor antibody [EPR4012](ab108985); Overlay histogram showing Jurkat cells stained with ab108985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.