OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-VIM antibodies

Anti-VIM Antibody EPR3776

div

Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307358
  • Rabbit monoclonal antibody against Vimentin (clone EPR3776 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401165) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(10)
IF(2)
FC(1)
Assay(1)
spacer
Also for VIM (NM_003380)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.
Clone NameEPR3776 IsotypeIgG
Species ReactivityMouse, Rat, Human, Rhesus monkey ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; FC: 1:50; IHC-P: 1:250 - 1:500
Predicted MW Explanation 54 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens vimentin (VIM)
Alternative NameCTRCT30; HEL113
Database LinkNP_003371
Entrez Gene 7431 Human
Entrez Gene 22352 Mouse
Entrez Gene 81818 Rat
Entrez Gene 705289 Monkey
FunctionVimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
Related PathwayES Cell Differentiation/IPS

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Vimentin antibody [EPR3776]; All lanes : Anti-Vimentin antibody [EPR3776] at 1/1000 dilution.Lane 1 : Hela cell lysate.Lane 2 : A549 cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.
Assay Image
Other-Anti-Vimentin antibody [EPR3776](TA307358); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody [EPR3776]; Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma using TA307358 at 1/250 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody [EPR3776]; TA307358 staining Vimentin in rat skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed using formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6. Samples were then blocked using 1% BSA for 1 hour at 25°C and incubated with TA307358 at a 1/400 dilution for 16 hours at 25°.C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal..Membrane of adipose cell is positive for vimention. Outer shealth of hair follicle and follicular papilla, fibrocytes, endothelial cells and vascular smooth muscle cells are all stained with vimentin.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Vimentin antibody [EPR3776](TA307358); TA307358 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Vimentin antibody [EPR3776](TA307358); TA307358 showing positive staining in Normal lung tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Vimentin antibody [EPR3776](TA307358); TA307358 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Vimentin antibody [EPR3776](TA307358); TA307358 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Vimentin antibody [EPR3776](TA307358); TA307358 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776]; TA307358 showing positive staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776]; Fluorescent immunohistochemical analysis of paraffin-embedded human normal colon tissue using TA307358. Green- Vimentin red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776]; Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney tissue using TA307358. Green- Vimentin red-PI.
IF Image
Immunocytochemistry/ Immunofluorescence - Vimentin antibody [EPR3776]; Immunofluorescent staining of HeLa cells TA307358 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776]; TA307358 staining vimentin in human Schlemms Canal Endothelium cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.2% and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in DPBS) for 3 hours at 20°C. An undiluted Alexa Fluor?488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-Vimentin antibody [EPR3776]; Overlay histogram showing HeLa cells stained with TA307358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton X-100 used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title