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Anti-USP7 Antibody EPR4253
Also for USP7 (NM_003470)
|A synthetic peptide corresponding to residues in human USP7 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens ubiquitin specific peptidase 7 (herpes virus-associated) (USP7), transcript variant 1|
|USP7 (Ubiquitin-specific-processing protease 7) is a deubiquitinase that has been shown to regulate the p53-Mdm2 pathway (1). Cotransfection of p53 and USP7 stabilizes p53 through the removal of ubiquitin moieties from polyubiquitinated p53 (1). It also counteracts the destabilizing effect of Mdm2 by direct deubiquitination of p53 (2). Subsequently, USP7 has been shown to deubiquitinate Mdm2 and Mdmx, thereby stabilizing these proteins (2). USP7 interacts with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a tenfold higher affinity than TP53 and can compete with it for USP7 binding (3).|
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Western blot - HAUSP / USP7 antibody [EPR4253]; All lanes : Anti-HAUSP / USP7 antibody [EPR4253] at 1/1000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lane 4 : MCF7 cell lysate.Lane 5 : C6 cell lysate.Lane 6 : RAW 264.7 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 128 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAUSP / USP7 antibody [EPR4253]; TA307354, at 1/50, staining HAUSP / USP7 in Human colon tissue by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - HAUSP / USP7 antibody [EPR4253]; TA307354, at 1/100, staining HAUSP / USP7 in HeLa cells by Immunofluorescence.