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Anti-NCK2 Antibody EPR3333


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against NCK2(clone EPR3333)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401190) , 20ug Explanation
100ul $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human NCK2 was used as an immunogen.
Clone NameEPR3333 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:100 - 1:250
Predicted MW Explanation 43 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1
Alternative NameGRB4; NCKbeta
Database LinkNP_003572
Entrez Gene 8440 Human
Entrez Gene 17974 Mouse
Entrez Gene 316369 Rat
FunctionNCK2 is a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. NCK2 has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization.
Related PathwayDruggable Genome ErbB signaling pathwayAxon guidanceT cell receptor signaling pathwayPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Nck beta antibody [EPR3333]; All lanes : Anti-Nck beta antibody [EPR3333] at 1/1000 dilution.Lane 1 : Human spleen lysate.Lane 2 : Human fetal brain lysate.Lane 3 : SH-SY5Y lysate.Lane 4 : Human fetal kidney lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 43 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nck beta antibody [EPR3333]; Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using TA307351 at a dilution of 1/50
Immunocytochemistry/ Immunofluorescence - Nck beta antibody [EPR3333]; Immunofluorescent staining of HeLa cells using TA307351 at a dilution of 1/100


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