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Home Antibody All anti-COPS3 antibodies

Anti-COPS3 Antibody EPR3127

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307347
  • Rabbit monoclonal antibody against COPS3 (clone EPR3127 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418520) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the C-terminus of human COPS3 was used as an immunogen.
Clone NameEPR3127 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:10000 - 1:20000; IP: 1:40; FC: 1:20 - 1:100; ICC/IF: 1:250 - 1:500
Predicted MW Explanation 48 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens COP9 signalosome subunit 3 (COPS3), transcript variant 1
Alternative NameCSN3; SGN3
Database LinkNP_003644
Entrez Gene 8533 Human
Entrez Gene 26572 Mouse
Entrez Gene 287367 Rat
FunctionCOPS3 is a component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control (1, 2). Subunit 3 (COPS3) possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-B alpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation (3). COPS3 is suggested as a specific negative regulator of TNF-induced NF-kappaB activation pathways because its over-expression inhibits NF-kappaB activation (4).
Related PathwayStem cell - Pluripotency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - COPS3 antibody [EPR3127]; All lanes : Anti-COPS3 antibody [EPR3127] at 1/20000 dilution.Lane 1 : HT-29 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : SKBR-3 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 48 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - COPS3 antibody [EPR3127]; Immunofluorescent staining of HeLa cells using TA307347 at 1/250 dilution.
FC Image
Flow Cytometry-Anti-COPS3 antibody [EPR3127](TA307347); Overlay histogram showing HeLa cells stained with TA307347 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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