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Anti-NRP1 Antibody EPR3113
Also for NRP1 (NM_003873)
|A synthetic peptide corresponding to residues in the cytoplasmic region of human Neuropilin-1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000; IHC-P: 1:100; IP: 1:100; FC: 1:50; IHC-Fr: Use at an assay dependent dilution; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens neuropilin 1 (NRP1), transcript variant 1|
|BDCA4; CD304; NP1; NRP; VEGF165R|
|Neuropilin-1 is a type 1 membrane protein with three distinct functions. First, it can mediate cell adhesion via a heterophilic molecular interaction. Second, in neuronal cells, neurophilin-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional guidance of axons. NP-1 isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis and in the formation of certain neuronal circuits and in organogenesis outside the nervous system (1). The soluble NP-1 isoform 2 binds VEGF(165) and appears to inhibit its binding to cells (2). It may also induce apoptosis by sequestering VEGF(165). NP-1 may bind various members of the semaphorin family. Its expression has an adverse effect on blood vessel number and integrity (3). |
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Western blot - Neuropilin 1 antibody [EPR3113]; All lanes : Anti-Neuropilin 1 antibody [EPR3113] at 1/1000 dilution.Lane 1 : Human placenta lysate.Lane 2 : HUVEC cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : mouse heart tissue lysate.Lane 5 : mouse kidney tissue lysate.Lane 6 : rat heart tissue lysate.Lane 7 : rat kidney tissue lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP goat anti rabbit at 1/2000 dilution.Predicted band size : 103 kDa.Observed band size : 120 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuropilin 1 antibody [EPR3113]; Immunohistochemical analysis of paraffin-embedded human kidney tissue using TA307337 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuropilin 1 antibody [EPR3113]; TA307337 staining Neuropilin 1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4Â°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Neuropilin 1 antibody [EPR3113]; TA307337 staining Neuropilin 1 in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 1 hour at 27Â°C followed by incubation with the primary antibody, undiluted, for 14 hours at 4Â°C. An undiluted Cy3?conjugated donkey anti-rabbit was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Neuropilin 1 antibody [EPR3113]; ICC/IF image of TA307337 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4Â°C. The secondary antibody (green) was DyLight? 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43ÂµM.
Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113]; TA307337 staining Neuropilin 1 in the COS1 fibroblast-like cell line derived from monkey kidney tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24Â°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4Â°C. An Alexa Fluor?488-conjugated Goat anti-rabbit monoclonal(1/500) was used as the secondary antibody.
Flow Cytometry-Neuropilin 1 antibody [EPR3113](TA307337); Overlay histogram showing HepG2 cells stained with TA307337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.