OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-USP13 antibodies

Anti-USP13 Antibody EPR4348

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307330
  • Rabbit monoclonal antibody against USP13(clone EPR4348)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401292) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IF(1)
spacer
Also for USP13 (NM_003940)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to human USP13 was used as an immunogen.
Clone NameEPR4348 IsotypeTissue culture supernatant
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: Use a concentration of 10 ug/ml
BufferDoes not react with Rat. Is unsuitable for IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)
Alternative NameIsoT-3; ISOT3
Database LinkNP_003931
FunctionUSP13 (Ubiquitin carboxyl-terminal hydrolase 13) is involved in thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a 76-residue protein attached to proteins as an intracellular targeting signal (1). It is highly expressed in the ovary and testes (1).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - USP13 antibody [EPR4348]; All lanes : Anti-USP13 antibody [EPR4348] at 1/1000 dilution.Lane 1 : SH SY5Y cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : A375 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 97 kDa.Observed band size : 97 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-USP13 antibody [EPR4348]; ICC/IF image of TA307330 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA307330 at 10ug/ml overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title