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Anti-BAG2 Antibody EPR3568
Also for BAG2 (NM_004282)
|A synthetic peptide corresponding to residues in the C-terminus of human Bag-2 was used as immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens BCL2-associated athanogene 2 (BAG2)|
|BAG-2 is a member of a family of BAG-related proteins (BAG-2, BAG-3, BAG-4, BAG-5). BAG-family proteins may play a role in providing opportunities for specification and diversification of Heat Shock Protein (Hsp70) chaperone functions. Hsp70 family molecular chaperones play critical roles in protein folding and trafficking in all eukaryotic cells. BAG-1 binds the ATPase domains of Hsp70 and Hsc70, modulating their chaperone activity and functioning as a competitive antagonist of the co-chaperone Hip. BAG-2 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner (1). |
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Western blot - BAG2 antibody [EPR3568]; All lanes : Anti-BAG2 antibody [EPR3568] at 1/2000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 24 kDa.Observed band size : 24 kDa.
Immunocytochemistry/ Immunofluorescence - BAG2 antibody [EPR3568]; Immunofluorescence analysis of HeLa cells with 1/100 TA307311.