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Anti-TRAF4 Antibody EPR1729
Also for TRAF4 (NM_004295)
|A synthetic peptide corresponding to residues in human TRAF4 was used as an immunogen.|
||Tissue culture supernatant
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; FC: 1:10 - 1:100
|Does not react with Rat. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IHC-P or IP.
|Homo sapiens TNF receptor-associated factor 4 (TRAF4)|
|CART1; MLN62; RNF83|
Entrez Gene 9618 Human
Entrez Gene 22032 Mouse
|The TNF-receptor-associated factor (TRAF) family is a phylogenetically conserved group of scaffold proteins that link receptors of the Toll-like receptor (TLR)-IL-1R and TNF receptor family to signaling cascades, leading to the activation of NF-kappaB and mitogen-activated protein kinases. Furthermore, TRAF proteins serve as docking platforms for a variety of regulators of these signaling pathways, and they are themselves often regulated at the transcriptional and posttranslational level (1). The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. TRAF4 has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. Thus, TRAF4 is thought to be involved in the oxidative activation of MAPK8/JNK (2).|
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Western blot - TRAF4 antibody [EPR1729]; All lanes : Anti-TRAF4 antibody [EPR1729] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : SKBR-3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 54 kDa.
Flow Cytometry - Anti-TRAF4 antibody [EPR1729]; Overlay histogram showing HeLa cells stained with TA307309 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.