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Home Antibody All anti-ARHGDIA antibodies

Anti-ARHGDIA Antibody EPR3773

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307308
  • Rabbit monoclonal antibody against Rho-GDI Alpha(clone EPR3773)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401371) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
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Also for ARHGDIA (NM_004309)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Rho-GDI Alpha was used as an immunogen.
Clone NameEPR3773 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
Predicted MW Explanation 23 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2
Alternative NameGDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1
Database LinkNP_004300
Entrez Gene 396 Human
Entrez Gene 192662 Mouse
Entrez Gene 360678 Rat
FunctionRho GDI constitutes a family with three mammalian members: Rho-GDI alpha, the ubiquitously expressed archetypal member of the family; Ly/D4-GDI or Rho-GDI beta, which has hematopoietic tissue-specific expression, particularly in B- and T-lymphocytes; and Rho-GDI-3 or -gamma, which is membrane-anchored through an amphipathic helix and is preferentially expressed in the brain, pancreas, lung, kidney and testis (1). RhoGDI’s function by extracting Rho family GTPases including RhoA, Rac1, and Cdc42 from membranes and solubilizing them in the cytosol. Moreover, they interact only with prenylated Rho proteins both in vitro and in vivo. They also inhibit nucleotide exchange and GTP hydrolyzing activities on Rho proteins by interacting with their switch regions and probably restricting accessibility to GEFs and GAPs (2). The N-terminal domain of RhoGDI alpha binds to the switch region of the GTPases, affecting GDP-GTP cycling, whereas the C-terminal domain accommodates the isoprenyl moiety of the GTPases in its hydrophobic pocket, regulating cytosol/membrane partitioning (3). Rho-GDI alpha can be phosphorylated on two sites, serine 101 and serine 174, by PAK (P21-activated kinase) (4).
Related PathwayDruggable Genome Neurotrophin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-RhoGDI antibody [EPR3773]; All lanes : Anti-RhoGDI antibody [EPR3773] at 1/1000 dilution.Lane 1 : Jurkat cell lysate .Lane 2 : HeLa cell lysate .Lane 3 : NIH3T3 cell lysate .Lysates/proteins at 10 ug per lane.Predicted band size : 23 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773]; Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RhoGDI with TA307308 at 1/100 dilution.

 

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