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Anti-CALR Antibody EPR3925
Also for CALR (NM_004343)
|A synthetic peptide corresponding to residues in human Calreticulin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens calreticulin (CALR)|
|cC1qR; CRT; RO; SSA|
|Calreticulin is a lectin-like, molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in the ER. Calreticulin participates in the synthesis of a variety of molecules such as ion channels, surface receptors, integrins and transporters (1). Calreticulin provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER (2). Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression (1).|
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Western blot - Calreticulin antibody [EPR3925]; All lanes : Anti-Calreticulin antibody [EPR3925] at 1/1000 dilution.Lane 1 : Fetal brain.Lane 2 : Fetal kidney.Lane 3 : NIH 3T3 cell lysates .Lysates/proteins at 10 µg per lane.Predicted band size : 48 kDa.Observed band size : 55 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calreticulin antibody [EPR3925]; Immunohistochemical staining of Calreticulin in paraffin embedded Human thyroid gland tissue, using ab108395 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Calreticulin antibody [EPR3925]; Immunofluorescent staining of Calreticulin in HeLa cells, using ab108395 at a 1/100 dilution.