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Home Antibody All anti-CALR antibodies

Anti-CALR Antibody EPR3924

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307304
  • Rabbit monoclonal antibody against Calreticulin(clone EPR3924)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401384) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(7)
IF(1)
FC(1)
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Also for CALR (NM_004343)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Calreticulin was used as an immunogen.
Clone NameEPR3924 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:10 - 100, ICC: 1:100 - 250, IHC: 1:250 - 500, IP: 1:10 - 100, WB: 1:1,000 - 10,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens calreticulin (CALR)
Alternative NamecC1qR; CRT; HEL-S-99n; RO; SSA
Database LinkNP_004334
FunctionCalreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters (1). Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER (2). Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression (1).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Calreticulin antibody [EPR3924]; All lanes : Anti-Calreticulin antibody [EPR3924] at 1/1000 dilution.Lane 1 : SH-SY5Y cell lysate.Lane 2 : HL-60 cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : HeLa cell lysate.Lane 5 : Fetal kidney lysate.Lane 6 : Fetal brain lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calreticulin antibody [EPR3924]; TA307304, at 1/250 dilution, staining Calreticulin in paraffin embedded Human kidney tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calreticulin antibody [EPR3924](TA307304); TA307304 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calreticulin antibody [EPR3924](TA307304); TA307304 showing negative staining in Normal heart tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calreticulin antibody [EPR3924](TA307304); TA307304 showing positive staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calreticulin antibody [EPR3924](TA307304); TA307304 showing positive staining in Normal placenta tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calreticulin antibody [EPR3924](TA307304); TA307304 showing positive staining in Normal stomach tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calreticulin antibody [EPR3924](TA307304); TA307304 showing positive staining in Papillary carcinoma of thyroid gland tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Calreticulin antibody [EPR3924]; TA307304, at 1/100 dilution, staining Calreticulin in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry-Anti-Calreticulin antibody [EPR3924](TA307304); Overlay histogram showing HeLa cells stained with TA307304 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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