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Anti-ERBB2 PHOSPHO Antibody EP2324Y
Also for ERBB2 (NM_004448)
|A phospho - specific peptide corresponding to residues surrounding tyrosine 877 of human HER2 was used as an immunogen. This antibody detects HER2 phosphorylated at tyrosine 877|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:500 - 1:1000; IHC-P: 1:250 - 1:500; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1|
|CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1|
Entrez Gene 2064 Human
|HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Autophosphorylation did not modulate receptor turnover. A Tyr----Phe substitution of ErbB-2 Tyr-877 homologous to pp60c-src Tyr-416 did not alter ErbB-2 biological and biochemical properties, thus excluding the possibility that phosphorylation of this residue, located in the kinase domain, modulates ErbB-2 gp185 catalytic function (4).|
|Protein KinaseTransmembraneDruggable Genome ErbB signaling pathwayCalcium signaling pathwayFocal adhesionAdherens junctionPathways in cancerPancreatic cancerMore Pathways >> |
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Western blot - ErbB 2 (phospho Y877) antibody [EP2324Y]; All lanes : Anti-ErbB 2 (phospho Y877) antibody [EP2324Y] at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : EGF treated HeLa cell lysate .Lane 3 : SKBR-3 cell lysate.Lane 4 : EGF treated SKBR-3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 138 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 2 (phospho Y877) antibody [EP2324Y]; TA307297, at 1/250, staining Human ErbB 2 (phospho Y877) in Human breast ductal infiltrating carcinoma tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - ErbB 2 (phospho Y877) antibody [EP2324Y]; TA307297, at 1/50 dilution, staining Human ErbB 2 (phospho Y877) in SKBR-3 cells by immunofluorescence.