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Anti-EZH2 PHOSPHO Antibody EPR1410
Also for EZH2 (NM_152998)
|A phospho specific peptide corresponding to residues surrounding threonine 487 of human Ezh 2 was used as an immunogen. This antibody only detects Ezh 2 phosphorylated at threonine 487.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 2|
|ENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2|
|Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo (2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3).|
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Western blot - KMT6 / EZH2 (phospho T487) antibody [EPR1410]; All lanes : Anti-KMT6 / EZH2 (phospho T487) antibody [EPR1410] at 1/1000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : HeLa cell lysates treated with LP.Lysates/proteins at 10 µg per lane.Predicted band size : 85 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KMT6 / EZH2 (phospho T487) antibody [EPR1410]; Immunohistochemical analysis of paraffin-embedded human tonsil tissue TA307294 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KMT6 / EZH2 (phospho T487) antibody [EPR1410]; Immunohistochemical analysis of paraffin-embedded human prostatic tissue using TA307294 at 1/100 dilution.