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Anti-YBX1 Antibody EP2706Y
Also for YBX1 (NM_004559)
|A synthetic peptide corresponding to residues near the N-terminus of human YBOX1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; IP: 1:50; FC: 1:60
|Does not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens Y box binding protein 1 (YBX1)|
|BP-8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1|
|Human DNA-binding proteins, dbpA and dbpB (YBOX-1), are members of a protein family containing a cold-shock domain, and are regarded as transcriptional regulators (1). The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor (2). Overexpression of YB-1 resulted in increased levels of PTP1B. Furthermore, depletion of YB-1 protein, by expression of a specific antisense construct, led to an approximately 70% decrease in expression of PTP1B, but no change in the level of its closest relative, TC-PTP. Data reveal an important role for YB-1 as a regulator of PTP1B expression, and further highlight PTP1B as a critical regulator of insulin- and cytokine-mediated signal transduction (3). |
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Western blot - YB1 antibody [EP2706Y]; All lanes : Anti-YB1 antibody [EP2706Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 36 kDa.Observed band size : 47 kDa .
Flow Cytometry - YB1 antibody [EP2706Y]; Flow cytometric analysis of permeabilized HeLa cells using ab76540 at 1/60 dilution (red) or a rabbit IgG (negative) (green)