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Anti-Nphs1 PHOSPHO Antibody EPTPG1
Also for Nphs1 (NM_004646)
|A synthetic peptide corresponding to residues surrounding tyrosine 1176 and 1193 of mouse Nephrin was used an immunogen. This antibody detects both Nephrin phosphorylated on tyrosines 1176 and 1193 as well as non-phosphorylated Nephrin.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)|
|CNF; nephrin; NPHN|
|Nephrin (NPHS1) is an immunoglobulin family adhesion protein expressed in visceral glomerular epithelial cells (podocytes), where it is targeted to the intercellular slit diaphragm of mature foot processes (1). It is an essential component of the kidney filtration barrier, and mutations in the nephrin gene are associated with congenital nephrotic syndrome (2). Nephrin binds via phosphorylated tyrosine residues in the intracellular domain to SH2 domain-containing signaling molecules such as Nck (3).|
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Western blot - Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]; All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] at 1/50000 dilution.Lane 1 : HEK293T cells transfected with wild type CD 16/7 Nephrin.Lane 2 : HEK293T cells transfected with active tyrosine kinase (vSrc).Lane 3 : HEK293T cells transfected with Nephrin mutated at Tyr1176 and vSrc.Lane 4 : HEK293T cells transfected with Nephrin mutated at Tyr 1193 and vSrc using Phospho Nephrin (pY1176/1193).Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 138 kDa.Observed band size : 138,180 kDa .