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Anti-ATG5 Antibody EPR1755(2)
Also for ATG5 (NM_004849)
|A synthetic peptide corresponding to residues in human Atg5 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens autophagy related 5 (ATG5), transcript variant 1|
|APG5; APG5-LIKE; APG5L; ASP; hAPG5|
|Atg5 (Autophagy protein 5) is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Atg5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity (1).|
Senescence and Autophagy
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Western blot - APG5L/ATG5 antibody [EPR1755(2)]; All lanes : Anti-APG5L/ATG5 antibody [EPR1755(2)] at 1/1000 dilution.Lane 1 : Raji cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : HT-1080 cell lysate.Lane 4 : Human fetal kidney cell lysate.Lane 5 : C6 cell lysate.Lane 6 : Raw264.7 cell lysate.Lane 7 : PC-12 cell lysate.Lane 8 : NIH3T3 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 32 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APG5L/ATG5 antibody [EPR1755(2)]; ab108327, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human hepatocellular carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APG5L/ATG5 antibody [EPR1755(2)]; ab108327, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry.