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Home Antibody All anti-MTOR antibodies

Anti-MTOR PHOSPHO Antibody EPR426(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307262
  • Rabbit monoclonal antibody against mTOR/FRAP Phospho (pS2448)(clone EPR426(2)) (Phospho-Specific)
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for MTOR (NM_004958)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP was used as an immunogen. This antibody only detects mTOR/FRAP phosphorylated at serine 2448.
Clone NameEPR426(2) IsotypeIgG
Species ReactivityMouse, Rat, Human, Pig ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)
Alternative NameFRAP; FRAP1; FRAP2; RAFT1; RAPT1
Database LinkNP_004949
Entrez Gene 2475 Human
Entrez Gene 56717 Mouse
Entrez Gene 56718 Rat
FunctionThe mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-terminal half of 20 tandem HEAT repeats that are implicated in protein-protein interactions and a C-terminal half that includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity, and the deletion of a single amino acid from this domain abrogates this activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the mRNA translation repressor, 4E-BP1, and also through the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Moreover, other known mediators of mTOR include PI3-K and ATK from the insulin pathway.
Related PathwayProtein KinaseDruggable Genome ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayType II diabetes mellitusPathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - mTOR (phospho S2448) antibody [EPR426(2)]; All lanes : Anti-mTOR (phospho S2448) [EPR426(2)] antibody at 1/1000 dilution.Lane 1 : 293T cell lysate, untreated.Lane 2 : 293T cell lysate, treated with Alkaline Phosphatase .Lysates/proteins at 10 ug per lane.Predicted band size : 289 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody [EPR426(2)]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using TA307262 at a dilution of 1/50.
IF Image
Immunocytochemistry/ Immunofluorescence - mTOR (phospho S2448) antibody [EPR426(2)]; Immunofluorescent staining of HeLa cells using TA307262 at a dilution of 1/100.
FC Image
Flow Cytometry - Anti-mTOR (phospho S2448) antibody [EPR426(2)]; Overlay histogram showing HeLa cells stained with TA307262 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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