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Home Antibody All anti-ISG15 antibodies

Anti-ISG15 Antibody EPR3446

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307251
  • Rabbit monoclonal antibody against ISG15(clone EPR3446)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417516) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for ISG15 (NM_005101)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human ISG15 was used as an immunogen.
Clone NameEPR3446 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens ISG15 ubiquitin-like modifier (ISG15)
Alternative NameG1P2; hUCRP; IFI15; IP17; UCRP
Database LinkNP_005092
Entrez Gene 9636 Human
FunctionISG15 is an ubiquitin like protein that is covalently conjugated to cellular proteins after IFN-alpha/beta stimulation (2). ISG15 is conjugated to their targets by an enzymatic cascade involving an E1 activating enzyme, an E2 conjugating enzyme, and in some cases an E3 ligase. ISG15 conjugation plays an important role by impacting nuclear and cytoplasmic functions as well as in antiviral response because a human virus, influenza B virus, inhibits ISG15 conjugation. However, the biological function of ISG15 modification remains unknown, largely because only a few human ISG15 target proteins have been identified (3).
Related PathwaySecreted ProteinDruggable Genome RIG-I-like receptor signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-ISG15 antibody [EPR3446]; All lanes : Anti-ISG15 antibody [EPR3446] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Daudi cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 18 kDa.Observed band size : 15 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody [EPR3446]; ImmunImmunofluorescent analysis of ISG15 in HeLa cells stained with TA307251 at a 1/100 dilution.

 

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