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Home Antibody All anti-HSPA5 antibodies

Anti-HSPA5 Antibody EPR4040(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307226
  • Rabbit monoclonal antibody against GRP78(clone EPR4040(2))
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417364) , 20ug
100ul 325 In Stock
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WB(2)
IHC(2)
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Also for HSPA5 (NM_005347)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human GRP78 was used as an immunogen.
Clone NameEPR4040(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5)
Alternative NameBIP; GRP78; HEL-S-89n; MIF2
Database LinkNP_005338
FunctionGRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell (1).
Related Pathway
Hemostasis
MAPK signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - GRP78 BiP antibody [EPR4040(2)]; All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : LnCaP cell lysate.Lane 3 : ECV-304 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 72 kDa.Observed band size : 78 kDa .
WB Image
Western blot - GRP78 BiP antibody [EPR4040(2)]; All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] at 1/1000 dilution.Lane 1 : C6 cell lysate.Lane 2 : NIH/3T3 cell lysate.Lane 3 : PC-12 cell lysate.Lane 4 : RAW264.7 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 72 kDa.Observed band size : 78 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)]; Immunohistochemical analysis of paraffin-embedded Human brain tissue using TA307226 at 1/250
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)]; Immunohistochemical analysis of paraffin-embedded Human testis tissue using TA307226 at 1/250

 

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