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Anti-CAMK2G Antibody EPR1828
Also for CAMK2G (NM_001222)
|A synthetic peptide corresponding to residues near the N-terminal of human CaMKII was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 4|
|CAMK; CAMK-II; CAMKG|
Entrez Gene 818 Human
Entrez Gene 12325 Mouse
Entrez Gene 171140 Rat
|Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. It is a ubiquitous serine/threonine protein kinase which is abundant in brain as a major PSD constituent. This enzyme is composed of distinct but related subunits (alpha, beta, gamma, and delta) (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2). |
|Protein KinaseDruggable Genome ErbB signaling pathwayCalcium signaling pathwayOocyte meiosisWnt signaling pathwayLong-term potentiationNeurotrophin signaling pathwayMore Pathways >> |
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Western blot - CaMKII alpha antibody [EPR1828]; All lanes : Anti-CaMKII alpha antibody [EPR1828].Lane 1 : Rat brain lysate.Lane 2 : SH SY5Y cell lysate.Lane 3 : Neuro 2a cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.
Flow Cytometry - Anti-CaMKII alpha antibody; Overlay histogram showing SH-SY5Y cells stained with TA307225 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in SH-SY5Y cells under the same conditions.