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Anti-MPL Antibody EPR4194
|A synthetic peptide corresponding to residues in human TPOR was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens myeloproliferative leukemia virus oncogene (MPL)|
|C-MPL; CD110; MPLV; THCYT2; TPOR|
|TPOR is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated (1).|
Cytokine-cytokine receptor interaction
Jak-STAT signaling pathway
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Western blot - TPOR antibody [EPR4194]; All lanes : Anti-TPOR antibody [EPR4194] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : A431 cell lysate.Lane 3 : Human marrow lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 71 kDa.
Flow Cytometry - Anti-TPOR antibody [EPR4194]; Overlay histogram showing MOLT4 cells stained with TA307223 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.