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Anti-MPL Antibody EPR4194
|A synthetic peptide corresponding to residues in human TPOR was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
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Western blot - TPOR antibody [EPR4194]; All lanes : Anti-TPOR antibody [EPR4194] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : A431 cell lysate.Lane 3 : Human marrow lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 71 kDa.
Flow Cytometry - Anti-TPOR antibody [EPR4194]; Overlay histogram showing MOLT4 cells stained with TA307223 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.